While PCR tests are highly effective, they also have some limitations: - Cost: PCR tests can be expensive compared to other diagnostic methods. - Technical Expertise: Requires specialized equipment and trained personnel. - False Positives/Negatives: Contamination or improper sample handling can lead to inaccurate results. - Not Quantitative: Standard PCR tests are not always quantitative, although real-time PCR (qPCR) can provide quantification.